Fig. 3

Effective knockdown of PD-L1 by siRNA in HTR-8/SVneo cells. HTR-8/SVneo cells were pre-incubated with PD-L1 siRNA for 48 h, and the levels of PD-L1 (a), p-ERK (b), MMP-2 (c) and MMP-9 (d) in NC and siRNA groups were detected by immunofluorescence; e The protein expression of PD-L1, MMP-2, MMP-9, ERK1/2 and pERK1/2 were evaluated by western blot in transfected cells. f Statistical analysis of the western blot results (** P < 0.01, *** P < 0.001)