Relationships between AFC and AMH concentrations in plasma, follicular fluid, and IVG media. a: AMH plasma concentrations were measured on the representative days of each stage of follicular growth (selection; day 4, luteal; day 13, ovulatory; day 6). AMH concentrations in follicular fluid at each stage of follicular growth were measured using the same sample as steroid hormones. We compared AMH concentrations between groups and stages of follicular growth. b: AMH concentrations in IVG media of OCGCs were measured. AMH concentrations were compared between groups and days of culture (days 4, 8, and 12)
The results of a factorial analysis by a two-way ANOVA were shown above each panel.
* An asterisk indicates a significant difference between the low and high AFC groups (P < 0.05).
†A dagger indicates a difference between the low and high AFC groups (P = 0.05).
a, b Different letters indicate significant differences between each day (P < 0.05).
Numbers in parentheses indicate the number of cows (a) or number of OCGCs (b). Error bars indicate SEM.