Relationships between AFC and the steroidogenesis of OCGCs during an IVG culture
Steroid hormones in the IVG media of OCGCs on days 4, 8, and 12 of culture were measured, and the production of E2 and P4 and the E2/P4 ratio were calculated as described in a previous study . We compared E2 and P4 concentrations and the E2/P4 ratio in IVG media between groups and the day of culture. The results of a factorial analysis by a two-way ANOVA were shown above each panel. a-c: Different letters indicate significant differences between different culture periods in the same group (P < 0.05). * An asterisk indicates a significant difference between the low and high AFC groups (P < 0.05). Numbers in parentheses indicate the number of OCGCs on the same day. Error bars indicate SEM.