Relationship between AFC and steroidogenesis in follicular fluid. Steroid hormones in follicular fluid collected from the largest follicles in each stage of follicular growth (selection, luteal, ovulatory) were measured, and we compared the concentration of each steroid hormone and the E2/P4 ratio in follicular fluid between groups and stages of follicular growth
The results of a factorial analysis by a two-way ANOVA were shown above each panel.
* An asterisk indicates a significant difference between the low and high AFC groups (P < 0.05).
a, b Different letters indicate significant differences between each stage (P < 0.05). Numbers in parentheses indicate the number of cows. Error bars indicate SEM.