Relationship between AFC and FSH, E2, T, and P4 plasma concentrations. A: FSH and steroid hormones were measured from days 0 to 16, and we compared the plasma concentration of each hormone between groups and days after follicular ablation. White arrowheads indicate the timing of follicular ablation and sampling of follicular fluid. B: Two to four days after follicular ablation was defined as the selection phase of follicles. The selection phases in two follicular waves after follicular ablation were pooled, and we compared the plasma concentration of each hormone between groups and days after follicular ablation during that period. The results of a factorial analysis by a two-way ANOVA were shown above each panel
a, b Different letters indicate significant differences between each day (P < 0.05).
Numbers in parentheses indicate the number of cows (a) or number of follicular waves (2 waves for each animal) (b). Error bars indicate SEM.