Fig. 2

Relationship between AFC and follicular growth dynamics monitored by ultrasonography. a: The number of follicles after the first follicular ablation were monitored by ultrasonography. The diameters of each follicle were measured. Follicles were classified into 3 groups according to their diameters (small: < 4 mm, intermediate: 4–8 mm, and large: ≥8 mm). We compared the number of antral follicles in each category between groups and days after follicular ablation. White arrowheads indicate the timing of follicular ablation and sampling of follicular fluid. b: The number of small, intermediate, and large antral follicles in two follicular waves from 1 to 6 days after follicular ablation (days 0 and 6) were pooled, and we compared the number of antral follicles between groups and days after follicular ablation

The results of a factorial analysis by a two-way ANOVA were shown above each panel.

^{a, b} Different letters indicate significant differences between each day (P < 0.05).

Numbers in parentheses indicate the number of cows (a) or number of follicular waves (2 waves for each animal) (b). Error bars indicate the standard error of the mean (SEM).