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Fig. 2 | Reproductive Biology and Endocrinology

Fig. 2

From: Contraceptive efficacy of sperm agglutinating factor from Staphylococcus warneri, isolated from the cervix of a woman with inexplicable infertility

Fig. 2

a Estimation of molecular weight of recombinant SAF. Lane 1: Pink plus Protein molecular weight marker; Lane 2: Purified recombinant SAF. b Inhibition of Mg++ dependent ATPase activity of human spermatozoa and mouse spermatozoa upon incubation with rSAF (0-50 μg). The results shown are mean ± S.D. of three observations, #p < 0.001. (H:human; M:mouse) (c) Visualization of FITC labeled recombinant rSAF incubated with human and d mouse spermatozoa by fluorescent microscopy (400X) showing rSAF binding to spermatozoa

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