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Fig. 6 | Reproductive Biology and Endocrinology

Fig. 6

From: Proteomic alterations underlie an association with teratozoospermia in obese mice sperm

Fig. 6

Diagnostic sperm parameters of normal and overweight or obese individuals and reduced abundance of CSPP1 and CETN1 in sperm from these individuals. a-d Body mass index (BMI) (a), sperm motility (b), sperm concentration (c) and sperm deformity (d) were calculated in normal (BMI < 25, n = 82) and overweight or obese (BMI ≥ 25, n = 190) individuals respectively. Data are expressed as mean ± STD. **P < 0.01. e Sperm morphology evaluated by Diff-Quick staining. Arrows indicate the abnormal sperm. Scale bars =10 μm. f CSPP1 and CETN1 localization in human sperm evaluated by immunofluorescent staining. Arrows indicated the positive signals in spermatozoa. Scale bars = 5 μm. g Western blot analysis of CSPP1 and CETN1 in human sperm from normal and overweight or obese individuals, respectively. Densitometric analysis evaluated the relative protein levels by a ratio of CSPP1 or CETN1 to α-tubulin in eight independent samples, respectively. Data are expressed as mean ± STD. *P < 0.05

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