Fig. 6From: Proteomic alterations underlie an association with teratozoospermia in obese mice spermDiagnostic sperm parameters of normal and overweight or obese individuals and reduced abundance of CSPP1 and CETN1 in sperm from these individuals. a-d Body mass index (BMI) (a), sperm motility (b), sperm concentration (c) and sperm deformity (d) were calculated in normal (BMI < 25, n = 82) and overweight or obese (BMI ≥ 25, n = 190) individuals respectively. Data are expressed as mean ± STD. **P < 0.01. e Sperm morphology evaluated by Diff-Quick staining. Arrows indicate the abnormal sperm. Scale bars =10 μm. f CSPP1 and CETN1 localization in human sperm evaluated by immunofluorescent staining. Arrows indicated the positive signals in spermatozoa. Scale bars = 5 μm. g Western blot analysis of CSPP1 and CETN1 in human sperm from normal and overweight or obese individuals, respectively. Densitometric analysis evaluated the relative protein levels by a ratio of CSPP1 or CETN1 to α-tubulin in eight independent samples, respectively. Data are expressed as mean ± STD. *P < 0.05Back to article page