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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Curcumin protects against palmitic acid-induced apoptosis via the inhibition of endoplasmic reticulum stress in testicular Leydig cells

Fig. 3

Curcumin attenuates the PA-induced apoptosis and ER stress in MLTC-1 cells. Cells were treated with different concentrations of curcumin (5–40 μM) in the absence (a) and presence (b) of PA (400 μM) for 24 h and then processed for cell activity analysis CCK 8 assay. Caspase-3 activity was measured using the Caspase-3 Activity Colorimetric Assay (c). Western blot analysis (e) was performed to detect the relative expression of apoptosis-related BAX (d) and ER stress marker GRP78 (f) and CHOP (g). The proteins expression levels were normalized to β-actin. The statistical analysis results are shown in the bar graphs. The data are represented as the mean ± SEM of three independent experiments. Bars with different letters are significantly different (p < 0.05)

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