Fig. 5
From: Downregulation of miR-322 promotes apoptosis of GC-2 cell by targeting Ddx3x
Changes in mir-424 and Ddx3x in the sperm plasma of males with high DFI infertility. a The relative expression of miR-424 was tested by quantitative RT-PCR using U6 as the internal control.miR-424 expression in seminal plasma of the group with high DFI male infertility was significantly reduced (1.14 vs 3.26). b The relative expression of Ddx3x was tested by quantitative RT-PCR using U6 as the internal control. c The relative expression of Ddx3x at the protein level was determined by Western blot using GAPDH as the internal control. b (3.14 vs 0.95) and c (2.1 or 2.4 vs 1) also reveal that, as the target gene locus of mir-322 in mice and the most likely target gene locus of mir-424 in the seminal plasma of male infertility patients with high DFI, Ddx3x showed a significant increase in mRNA (3.14 vs 0.95) and protein (2.1 or 2.4 vs 1) levels . All data represent the mean ± SEM of at least three independent experiments (*P < 0.05, ** P < 0.01)