Table 1 Specific primer sequences to detect gene expression in llama in granulosa cells
Gene | ID | 5′-3’ | Length (bp) | product (bp) |
|---|---|---|---|---|
3-beta-hydroxy steroid dehydrogenase (HSD3B) | LL-HSD3B-F | TGGTGGGCTTCTTGCTTAGT | 20 | 174 |
LL-HSD3B-R | CAACCCATTCCGTGGTATTC | 20 | ||
Steroidogenic acute regulatory protein (StAR) | LL-STAR-F | GAATGGGGACGAAGTGCTAA | 20 | 200 |
LL-STAR-R | GCTCATGGGTGATGACTGTG | 20 | ||
Side-Chain cleavage enzyme (p450scc) | LL-p450scc-F | GCCACTGCTCTTCCTGTCAT | 20 | 237 |
LL-p450scc-R | GCCATTTATTGCCTTCATGG | 20 | ||
Aromatase (p450 Arom) | LL-p450arom-F | GTGTCCGGAGTGTGCCTGTT | 21 | 148 |
LL-p450arom-R | GGAACCTGCAGTGGGAAATGA | 21 | ||
Vascular endothelial growth factor (VEGF) | LL-VEGF-F | TCCAATCGAGACCCTGGTAG | 20 | 168 |
LL-VEGF-R | ATCCGCATAATCTGCATGGT | 20 | ||
Large ribosomal protein (RPLP0) | rplp0-F | GGCGACCTGGAAGTCCAACT | 20 | 149 |
rplp0-R | CCATCAGCACCACAGCCTTC | 20 |