Fig. 4From: Entry into puberty is reflected in changes in hormone production but not in testicular receptor expression in Atlantic salmon (Salmo salar)Immunocytochemical detection of proliferation activity in Atlantic salmon testis. The proliferation marker phosphorylated histone H3 (pH 3; brown staining in nuclei) and hematoxylin counterstain (light blue) of testis sections from males of experiment 2 in the SGA stage. pH 3-positive germ cell (arrows) and Sertoli cell (asterisks) nuclei were counted; pH 3 staining in the interstitial area was disregarded. Males in the SGA stage showed a low (a; immature; n = 24) or an elevated (b; maturing; n = 11) number of pH 3-positive nuclei (mean ± S.E.M. per viewfield at 400-fold magnification) that differed significantly (p < 0.0001; Student t-test). Bars indicate 30 μmBack to article page