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Fig. 4 | Reproductive Biology and Endocrinology

Fig. 4

From: Entry into puberty is reflected in changes in hormone production but not in testicular receptor expression in Atlantic salmon (Salmo salar)

Fig. 4

Immunocytochemical detection of proliferation activity in Atlantic salmon testis. The proliferation marker phosphorylated histone H3 (pH 3; brown staining in nuclei) and hematoxylin counterstain (light blue) of testis sections from males of experiment 2 in the SGA stage. pH 3-positive germ cell (arrows) and Sertoli cell (asterisks) nuclei were counted; pH 3 staining in the interstitial area was disregarded. Males in the SGA stage showed a low (a; immature; n = 24) or an elevated (b; maturing; n = 11) number of pH 3-positive nuclei (mean ± S.E.M. per viewfield at 400-fold magnification) that differed significantly (p < 0.0001; Student t-test). Bars indicate 30 μm

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