Fig. 2

Representative dot-plots from Fluorescence-Activated Cell Sorting of endothelial and steroidogenic cell fractions from bovine CL. A Dot-plots showing selection of single events (individual cells) by SSC-A vs SSC-H (a), which were then visualized as FSC-A vs SSC-A (b), and sorted (c, d) by selection for CD144+ staining (c1). CD144- and DAPI- cells (c2) were further analyzed to select cells with high Nile Red (Nile Red^Hi) fluorescence signal (d). B, C Control cells stained with CD144 (primary and secondary) antibodies (B) and Nile Red (C) for detection of CD144 (Ba) and Nile Red (Cb) signals. “B” and “YG” in the X and Y axis stand for Blue and Yellow-Green laser, respectively. DAPI was used for live/dead cell labelling