Fig. 1
From: Seasonal variation in equine follicular fluid proteome
Follicular fluid (FF) protein isolation through combined precipitation and depletion approach. Representative gel electrophoresis of equine FF submitted to four different Acetone-TCA-based protein precipitation protocols (a), followed by albumin depletion (b) are shown. Gels were stained with Coomassie blue to visualize the protein bands, showing decreased protein amounts following both precipitation and depletion. Utilization of pure FF revealed higher protein recovery following depletion. Extraction protocols (5:4:1, 3.4:6.6:0, and 2:8:0) corresponded to FF:Acetone:TCA, respectively