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Fig. 5 | Reproductive Biology and Endocrinology

Fig. 5

From: Activation of Nrf2/Keap1 pathway by oral Dimethylfumarate administration alleviates oxidative stress and age-associated infertility might be delayed in the mouse ovary

Fig. 5

mRNA and protein levels of Nrf2 and antioxidants. Effect of DMF administration on the mRNA levels were investigated by qRT-PCR. The mRNA expression of Nrf2, catalase, SOD1, and NQO1 was normalized to RNA loading for each sample using GAPDH mRNA as an internal standard. DMF administration resulted in significant elevation of mRNA levels of Nrf2 (1.50-fold compared to control group, p < 0.05), catalase (1.91-fold compared to control group, p < 0.05), SOD1 (1.42-fold compared to control group, p < 0.05), and NQO1 (1.84-fold compared to control group, p < 0.05). The results are shown as the mean ± SD (bars) of 3 or 4 independent experiments. Simultaneously, DMF administration increased expression of Nrf2, catalase, SOD1, and NQO1 proteins in WB analysis. The protein levels of Keap1 was conversely decreased by DMF administration

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