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Table 3 Effect of low protein diet with and without supplemental folate on oocyte serine and cysteine kinetics and cumulus cell amino acid concentration, respectively, in rats

From: Preovulatory exposure to a protein-restricted diet disrupts amino acid kinetics and alters mitochondrial structure and function in the rat oocyte and is partially rescued by folic acid

  Control (n = 5) LP (n = 5) LPF (n = 6) P value
Oocyte kinetics (μmol·kg− 1·h− 1)
 Serine flux 2722 ± 215 ab 2133 ± 191 a 3130 ± 329 b 0.033
 Cysteine flux 355 ± 22.9 a 261 ± 25.8 b 255 ± 18.23 b 0.012
 TS 416 ± 32.2 274 ± 10.7 387 ± 56.6 0.0796
Cumulus cell amino acid concentration (nmol per nmol pellet phenylalanine)
 Methionine 0.019 ± 0.005 a 0.028 ± 0.008 ab 0.055 ± 0.014 b 0.0434
 Homocysteine 0.106 ± 0.015 0.173 ± 0.053 0.217 ± 0.042 0.136
 Cysteine 8.94 ± 2.07 9.45 ± 2.25 11.4 ± 3.00 0.765
 Serine 4.77 ± 0.89 8.45 ± 2.04 7.72 ± 1.53 0.213
 Glycine 7.45 ± 1.42 6.10 ± 0.91 3.68 ± 1.06 0.092
  1. Note: Data are mean ± SEM; LP = Low Protein; LPF = Low Protein with supplemental folate
  2. TS transsulfuration (conversion of serine to cysteine)
  3. Means with different superscript letters are significantly different, P < 0.05 (Tukey’s multiple comparison test)