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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Maternal high-fat diet impairs follicular development of offspring through intraovarian kisspeptin/GPR54 system

Fig. 3

Effects of HFD during gestation in follicular development at prepuberty in the female offspring. Representative pictures of prepuberty ovaries (PND30) from control group (a) and HFD group (b) (40X). c and d corresponds to magnification of the a and b (100X). The count results of various structures were shown in i (n = 4). Representative photomicrographs of kisspeptin (e, f) and GPR54 (g, h) immunostaining of PND30 offspring ovaries from NCD and HFD fed dams. And the MOD for kisspeptin and GPR54 were summarized in j (n = 5–7). Quantification of the levels of mRNA to follicular growth-related genes (k), ovulation-related genes (l), steroidogenesis synthesis related genes (m) and kiss1/kiss1r (n) by RT-qPCR. values are the means ± S.E.M (n = 5–8). *P < 0.05 HFD vs NCD

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