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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Profile of melatonin and its receptors and synthesizing enzymes in cumulus–oocyte complexes of the developing sheep antral follicle—a potential estradiol-mediated mechanism

Fig. 3

Effect of E2 and ER antagonist ICI182780 on melatonin production and the expression of AANAT, HIOMT, MT1, and MT2 mRNA and protein in sheep COCs. a Melatonin levels (n = 6 per group). b The relative expression of AANAT, HIOMT, MT1, and MT2 mRNA. Independent experiments were performed three times, with β-actin as an internal control. c Western blotting of AANAT, HIOMT, MT1, MT2 and the relative expression of AANAT, HIOMT, MT1, and MT2 protein in each COCs treatment group. β-actin was used as an internal control. Different superscript letters (a-c) indicate statistically significant differences (P < 0.05)

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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