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Fig. 2 | Reproductive Biology and Endocrinology

Fig. 2

From: Profile of melatonin and its receptors and synthesizing enzymes in cumulus–oocyte complexes of the developing sheep antral follicle—a potential estradiol-mediated mechanism

Fig. 2

Relative expression of AANAT, HIOMT, MT1, and MT2 in COCs of follicles of different sizes. a The relative expression of AANAT, HIOMT, MT1, and MT2 mRNA. Independent experiments were performed three times, with β-actin as an internal control. b Western blotting of AANAT, HIOMT, MT1, MT2 and the relative expression of AANAT, HIOMT, MT1, and MT2 protein in COCs of follicles of different sizes. β-actin was used as an internal control. Data are means ± SEM. Different superscript letters (a-c) indicate statistically significant differences (P < 0.05). S: small follicle; M: medium follicle; L: large follicle

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