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Fig. 5 | Reproductive Biology and Endocrinology

Fig. 5

From: Expression of transcriptional factor EB (TFEB) in differentiating spermatogonia potentially promotes cell migration in mouse seminiferous epithelium

Fig. 5

Knock down of TFEB by shRNA lentiviral transduction in GC-1 cells. a The lentiviral infection efficiency was accessed by detection of the EGFP using flow cytometer analysis. After lentiviral transduction for 48 h and 72 h, shRNA lentiviral expression efficiency estimated at 94 ± 3% and 90 ± 6%, respectively (n = 4). b The relative mRNA levels of TFEB after lentiviral transduction for 48 h and 72 h were 0.35 ± 0.11 and 0.40 ± 0.09, comparing to the controls (n = 6, ** p < 0.01). c Cell survival assay performed with MTS method, showed no significant difference after shRNA lentiviral infection for 48 h and 72 h (n = 6)

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