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Fig. 2 | Reproductive Biology and Endocrinology

Fig. 2

From: miR-34c disrupts spermatogonial stem cell homeostasis in cryptorchid testes by targeting Nanos2

Fig. 2

miR-34c inhibit the expression of Nanos2 in GC-1 cells. a Relative expression levels of miR-34c and Nanos2 mRNA were analyzed by RT-qPCR after transfecting the plasmid of miR-34c in GC-1 cell. b Relative expression levels of miR-34c and Nanos2 mRNA were analyzed by RT-qPCR after miR-34c inhibition in GC-1 cell. c Nanos2 protein levels in a GC-1 cell culture after miR-34c overexpression or inhibition. Con: control; NC: negative control. *P < 0.05, **P < 0.01 and ***P < 0.001

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