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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Rosiglitazone ameliorates palmitic acid-induced cytotoxicity in TM4 Sertoli cells

Fig. 3

Knockdown of PPARγ alleviated the protective effects of RSG on PA-induced lipotoxicity in Sertoli cells. TM4 cells were transfected with NC-siRNA or siPPARγ. 6 h after transfection, cells were pretreated with (or without) RSG for 2 h, and then treated with PA for 24 h. a MTT assay of TM4 cells. b and c ORO staining of primary mouse Sertoli cells (b) and quantification of neutral lipids (c). Data are presented as the mean ± standard deviation of three independently prepared samples, each with three measurements. Scale bar, 100 μm. **P < 0.01 vs. control group; ##P < 0.01 vs. 0.4 mM-PA group; &P < 0.05 vs. 0.4 mM-PA + 20 μM-RSG group. RSG rosiglitazone, PA palmitic acid, ORO oil red O

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