Fig. 1

RSG ameliorates the PA-induced decrease in Sertoli cell viability. (a and b) An MTT assay was performed with TM4 cells treated with PA for (a) 12 h or (b) 24 h, with or without RSG pre-treatment. c Dose- and time-dependent analysis of the effect of RSG on PA-induced cytotoxicity (MTT assay). d An MTT assay was conducted in primary mouse sertoli cells treated with PA for 24 h with or without RSG pre-treatment. Data are presented as the mean ± standard deviation of three independently prepared samples, each with three measurements. ^*P < 0.05 and ^**P < 0.01 vs. control group; ^$P < 0.05 vs. 0.2 mM-PA group; ^#P < 0.05 and ^##P < 0.01 vs. 0.4 mM-PA group. RSG rosiglitazone, PA palmitic acid, MTT 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide