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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Alleviation of endoplasmic reticulum stress protects against cisplatin-induced ovarian damage

Fig. 3

Suppressing autophagy hardly alleviates CDDP-induced cell apoptosis or ERS. a Autophagy levels in KGN cells after indicated treatments by DALGreen staining. Positive autophagosomes were observed by fluorescence microscopy. White arrow indicates autophagosome-negative cell and the red indicates autophagsome-positive cell. Scale bars = 80 μm. b Quantification of the positive phagosomes in a. c KGN and COV434 cells were treated with 50 μM CDDP with/without 5 mM 3-MA for indicated times. ERS, autophagy and apoptosis levels were detected by western blotting. d KGN cells were treated with 50 μM CDDP with/without 5 mM 3-MA for 24 h and then stained with Annexin V-FITC and PI. e Quantification of the results in d. * P < 0.05

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