Skip to main content

Advertisement

Fig. 2 | Reproductive Biology and Endocrinology

Fig. 2

From: Alleviation of endoplasmic reticulum stress protects against cisplatin-induced ovarian damage

Fig. 2

Inhibition of ERS depresses CDDP-induced ERS, autophagy and apoptosis in granulosa cells. a Immunofluorescence of HSP90AB1 and HSPA5 in the saline and CDDP-treated ovaries. Nuclei were stained with DAPI. White arrow indicates primordial follicle; red, primary follicle; yellow, secondary follicle; green, antral follicle. Red scale bars = 80 μm, White scale bars = 10 μm (b) Immunoblotting of HSP90AB1 and HSPA5 in the KGN cells treated with CDDP at indicated concentrations (0, 2, 5, 10, 25 and 50 μM) for 24 h. c Immunoblotting of HSP90AB1 and HSPA5 in the KGN cells treated with CDDP at 50 μM for indicated times (0, 4, 8, 16, 24 h). d KGN and COV434 cells were transfected with HSPA5 and HSP90AB1-specific siRNA and NC-siRNA respectively for 48 h, and then treated with 50 μM CDDP for indicated times. Immunoblotting was carried out to detect the protein levels of ERS-, autophagy- and apoptosis-related genes. e KGN and COV434 cells were treated with 50 μM CDDP with/without 5 mM 4-PBA for indicated times. ERS, autophagy and apoptosis levels were detected by western blot. f KGN cells were treated with 50 μM CDDP with/without 5 mM 4-PBA for 24 h and then stained with Annexin V-FITC and PI. Both cells at early (annexin Vpos; PIneg) and late apoptotic stages (annexin Vpos; PIpos) were counted. g Quantification of the results in f. * P < 0.05, ** P < 0.01

Back to article page