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Table 8 Summary of Rs values for induced AR assay
| Subgroup | N | Rs (95% CI) | Test(s) of heterogeneity | Significance test(s) of Rs = 0 | P a | ||
|---|---|---|---|---|---|---|---|
| I2 (%) | P | Z | P | ||||
| Overall | 17 | 0.40 (0.24–0.57) | 96.5 | 0.000 | 4.87 | 0.000 | |
| Three experiments excluded | 14 | 0.36 (0.24–0.47) | 83.6 | 0.000 | 5.99 | 0.000 | |
| Geographic area | |||||||
| Europe | 10 | 0.33 (0.11–0.55) | 95.7 | 0.000 | 2.98 | 0.003 | < 0.001 |
| Oceania | 2 | 0.40 (0.03–0.76) | 89.0 | 0.003 | 2.11 | 0.035 | |
| South America | 1 | 0.46 (0.17–0.75) | –b | – | 3.14 | 0.002 | |
| Africa | 2 | 0.65 (0.05–1.25) | 97.1 | 0.000 | 2.12 | 0.034 | |
| North America | 2 | 0.49 (0.30–0.69) | 57.8 | 0.124 | 4.95 | 0.000 | |
| Preparation method | |||||||
| No preparation | 1 | 0.39 (0.20–0.58) | – | – | 4.12 | 0.000 | < 0.001 |
| One preparation | 15 | 0.41 (0.24–0.58) | 96.7 | 0.000 | 4.63 | 0.000 | |
| Swim up | 6 | 0.65 (0.49–0.81) | 93.0 | 0.000 | 8.07 | 0.000 | < 0.001 |
| Swim up/discontinuous gradient | 2 | 0.61 (0.42–0.81) | 50.9 | 0.153 | 6.07 | 0.000 | |
| Discontinuous gradient | 7 | 0.17 (0.10–0.25) | 19.0 | 0.285 | 4.42 | 0.000 | |
| Double preparation | 1 | 0.33 (0.13–0.53) | – | – | 3.28 | 0.001 | |
| AR trigger | |||||||
| Physiological trigger | 8 | 0.49 (0.28–0.70) | 96.8 | 0.000 | 4.51 | 0.000 | < 0.001 |
| HFF | 3 | 0.46 (−0.03–0.95) | 97.3 | 0.000 | 1.85 | 0.065 | < 0.001 |
| P | 2 | 0.31(−0.01–0.63) | 82.4 | 0.017 | 1.89 | 0.059 | |
| ZP | 3 | 0.63 (0.25–1.01) | 96.0 | 0.000 | 3.22 | 0.001 | |
| Nonphysiological trigger | 9 | 0.33 (0.15–0.50) | 89.0 | 0.000 | 3.68 | 0.000 | |
| A23187 | 5 | 0.36 (0.13–0.58) | 89.1 | 0.000 | 3.12 | 0.002 | < 0.001 |
| CAMP | 1 | 0.12(−0.06–0.29) | – | – | 1.26 | 0.206 | |
| TPA | 1 | 0.03 (−0.15–0.21) | – | – | 0.29 | 0.773 | |
| BSA-GlcNAc/A23187 | 1 | 0.46 (0.17–0.75) | – | – | 3.14 | 0.002 | |
| Mannose | 1 | 0.59 (0.42–0.76) | – | – | 6.68 | 0.000 | |
| AR assay method | |||||||
| Fluorescent labels | 16 | 0.41 (0.25–0.58) | 96.5 | 0.000 | 4.89 | 0.000 | < 0.001 |
| Direct immunofluorescence | 15 | 0.40 (0.21–0.58) | 96.7 | 0.000 | 4.22 | 0.000 | 0.224 |
| With lection | 10 | 0.53 (0.36–0.70) | 94.9 | 0.000 | 6.08 | 0.000 | < 0.001 |
| FITC-PSA | 7 | 0.55 (0.36–0.75) | 95.7 | 0.000 | 5.57 | 0.000 | < 0.001 |
| FITC-PNA | 2 | 0.40 (0.23–0.58) | 18.3 | 0.269 | 4.51 | 0.000 | |
| RITC-PSA | 1 | 0.59 (0.42–0.76) | – | – | 6.68 | 0.000 | |
| With antibody | |||||||
| FITC-GB24 | 5 | 0.15 (0.05–0.25) | 40.3 | 0.152 | 2.93 | 0.003 | |
| Indirect immunofluorescence | |||||||
| Anti-CD46 antibody | 1 | 0.68 (0.59–0.77) | – | – | 14.14 | 0.000 | |
| Triple stain | 1 | 0.24 (0.03–0.45) | – | – | 2.24 | 0.025 | |
