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Table 8 Summary of Rs values for induced AR assay

From: Human sperm acrosome function assays are predictive of fertilization rate in vitro: a retrospective cohort study and meta-analysis

Subgroup N Rs (95% CI) Test(s) of heterogeneity Significance test(s) of Rs = 0 P a
I2 (%) P Z P  
Overall 17 0.40 (0.24–0.57) 96.5 0.000 4.87 0.000  
Three experiments excluded 14 0.36 (0.24–0.47) 83.6 0.000 5.99 0.000  
Geographic area
 Europe 10 0.33 (0.11–0.55) 95.7 0.000 2.98 0.003 < 0.001
 Oceania 2 0.40 (0.03–0.76) 89.0 0.003 2.11 0.035  
 South America 1 0.46 (0.17–0.75) b 3.14 0.002  
 Africa 2 0.65 (0.05–1.25) 97.1 0.000 2.12 0.034  
 North America 2 0.49 (0.30–0.69) 57.8 0.124 4.95 0.000  
Preparation method
 No preparation 1 0.39 (0.20–0.58) 4.12 0.000 < 0.001
 One preparation 15 0.41 (0.24–0.58) 96.7 0.000 4.63 0.000  
  Swim up 6 0.65 (0.49–0.81) 93.0 0.000 8.07 0.000 < 0.001
  Swim up/discontinuous gradient 2 0.61 (0.42–0.81) 50.9 0.153 6.07 0.000  
  Discontinuous gradient 7 0.17 (0.10–0.25) 19.0 0.285 4.42 0.000  
 Double preparation 1 0.33 (0.13–0.53) 3.28 0.001  
AR trigger        
 Physiological trigger 8 0.49 (0.28–0.70) 96.8 0.000 4.51 0.000 < 0.001
  HFF 3 0.46 (−0.03–0.95) 97.3 0.000 1.85 0.065 < 0.001
  P 2 0.31(−0.01–0.63) 82.4 0.017 1.89 0.059  
  ZP 3 0.63 (0.25–1.01) 96.0 0.000 3.22 0.001  
 Nonphysiological trigger 9 0.33 (0.15–0.50) 89.0 0.000 3.68 0.000  
  A23187 5 0.36 (0.13–0.58) 89.1 0.000 3.12 0.002 < 0.001
  CAMP 1 0.12(−0.06–0.29) 1.26 0.206  
  TPA 1 0.03 (−0.15–0.21) 0.29 0.773  
  BSA-GlcNAc/A23187 1 0.46 (0.17–0.75) 3.14 0.002  
  Mannose 1 0.59 (0.42–0.76) 6.68 0.000  
AR assay method        
 Fluorescent labels 16 0.41 (0.25–0.58) 96.5 0.000 4.89 0.000 < 0.001
  Direct immunofluorescence 15 0.40 (0.21–0.58) 96.7 0.000 4.22 0.000 0.224
   With lection 10 0.53 (0.36–0.70) 94.9 0.000 6.08 0.000 < 0.001
    FITC-PSA 7 0.55 (0.36–0.75) 95.7 0.000 5.57 0.000 < 0.001
    FITC-PNA 2 0.40 (0.23–0.58) 18.3 0.269 4.51 0.000  
    RITC-PSA 1 0.59 (0.42–0.76) 6.68 0.000  
   With antibody
    FITC-GB24 5 0.15 (0.05–0.25) 40.3 0.152 2.93 0.003  
  Indirect immunofluorescence
   Anti-CD46 antibody 1 0.68 (0.59–0.77) 14.14 0.000  
 Triple stain 1 0.24 (0.03–0.45) 2.24 0.025  
  1. Rs spearman correlation coefficient, AR acrosome reaction, CI confidence interval, HFF human follicle fluid, P progesterone, ZP zona pellucida, CAMP cyclic adenosine 3′-5′-phosphate analogue, TPA phorbol ester, BSA-GlcNAc Neoglycoproteins with N-acetylglucosamine residues, FITC-PSA fluorescein isothiocyanate-conjugated Pisurn sativum agglutimm, FITC-PNA fluorescein isothiocyanate-conjugated peanut agglutinin, RITC-PSA rhodamine-conjugated Pisurn sativum agglutimm
  2. aComparison between subgroups with the Q test for heterogeneity
  3. bNot calculated