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Table 8 Summary of Rs values for induced AR assay

From: Human sperm acrosome function assays are predictive of fertilization rate in vitro: a retrospective cohort study and meta-analysis

Subgroup

N

Rs (95% CI)

Test(s) of heterogeneity

Significance test(s) of Rs = 0

P a

I2 (%)

P

Z

P

 

Overall

17

0.40 (0.24–0.57)

96.5

0.000

4.87

0.000

 

Three experiments excluded

14

0.36 (0.24–0.47)

83.6

0.000

5.99

0.000

 

Geographic area

 Europe

10

0.33 (0.11–0.55)

95.7

0.000

2.98

0.003

< 0.001

 Oceania

2

0.40 (0.03–0.76)

89.0

0.003

2.11

0.035

 

 South America

1

0.46 (0.17–0.75)

–b

–

3.14

0.002

 

 Africa

2

0.65 (0.05–1.25)

97.1

0.000

2.12

0.034

 

 North America

2

0.49 (0.30–0.69)

57.8

0.124

4.95

0.000

 

Preparation method

 No preparation

1

0.39 (0.20–0.58)

–

–

4.12

0.000

< 0.001

 One preparation

15

0.41 (0.24–0.58)

96.7

0.000

4.63

0.000

 

  Swim up

6

0.65 (0.49–0.81)

93.0

0.000

8.07

0.000

< 0.001

  Swim up/discontinuous gradient

2

0.61 (0.42–0.81)

50.9

0.153

6.07

0.000

 

  Discontinuous gradient

7

0.17 (0.10–0.25)

19.0

0.285

4.42

0.000

 

 Double preparation

1

0.33 (0.13–0.53)

–

–

3.28

0.001

 

AR trigger

       

 Physiological trigger

8

0.49 (0.28–0.70)

96.8

0.000

4.51

0.000

< 0.001

  HFF

3

0.46 (−0.03–0.95)

97.3

0.000

1.85

0.065

< 0.001

  P

2

0.31(−0.01–0.63)

82.4

0.017

1.89

0.059

 

  ZP

3

0.63 (0.25–1.01)

96.0

0.000

3.22

0.001

 

 Nonphysiological trigger

9

0.33 (0.15–0.50)

89.0

0.000

3.68

0.000

 

  A23187

5

0.36 (0.13–0.58)

89.1

0.000

3.12

0.002

< 0.001

  CAMP

1

0.12(−0.06–0.29)

–

–

1.26

0.206

 

  TPA

1

0.03 (−0.15–0.21)

–

–

0.29

0.773

 

  BSA-GlcNAc/A23187

1

0.46 (0.17–0.75)

–

–

3.14

0.002

 

  Mannose

1

0.59 (0.42–0.76)

–

–

6.68

0.000

 

AR assay method

       

 Fluorescent labels

16

0.41 (0.25–0.58)

96.5

0.000

4.89

0.000

< 0.001

  Direct immunofluorescence

15

0.40 (0.21–0.58)

96.7

0.000

4.22

0.000

0.224

   With lection

10

0.53 (0.36–0.70)

94.9

0.000

6.08

0.000

< 0.001

    FITC-PSA

7

0.55 (0.36–0.75)

95.7

0.000

5.57

0.000

< 0.001

    FITC-PNA

2

0.40 (0.23–0.58)

18.3

0.269

4.51

0.000

 

    RITC-PSA

1

0.59 (0.42–0.76)

–

–

6.68

0.000

 

   With antibody

    FITC-GB24

5

0.15 (0.05–0.25)

40.3

0.152

2.93

0.003

 

  Indirect immunofluorescence

   Anti-CD46 antibody

1

0.68 (0.59–0.77)

–

–

14.14

0.000

 

 Triple stain

1

0.24 (0.03–0.45)

–

–

2.24

0.025

 
  1. Rs spearman correlation coefficient, AR acrosome reaction, CI confidence interval, HFF human follicle fluid, P progesterone, ZP zona pellucida, CAMP cyclic adenosine 3′-5′-phosphate analogue, TPA phorbol ester, BSA-GlcNAc Neoglycoproteins with N-acetylglucosamine residues, FITC-PSA fluorescein isothiocyanate-conjugated Pisurn sativum agglutimm, FITC-PNA fluorescein isothiocyanate-conjugated peanut agglutinin, RITC-PSA rhodamine-conjugated Pisurn sativum agglutimm
  2. aComparison between subgroups with the Q test for heterogeneity
  3. bNot calculated
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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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