|
Subgroup
|
N
|
Rs (95% CI)
|
Test(s) of heterogeneity
|
Significance test(s) of Rs = 0
|
P
a
|
|---|
|
I2 (%)
|
P
|
Z
|
P
| |
|---|
|
Overall
|
13
|
0.39 (0.18–0.60)
|
95.7
|
0.000
|
3.70
|
0.000
| |
|
Two experiments excluded
|
11
|
0.38 (0.27–0.48)
|
67.1
|
0.001
|
7.19
|
0.000
| |
|
Geographic area
|
|
Asia
|
4
|
0.24 (0.05–0.42)
|
66.7
|
0.029
|
2.49
|
0.013
|
< 0.001
|
|
North America
|
1
|
0.54 (0.43–0.65)
|
–b
|
–
|
9.70
|
0.000
| |
|
Europe
|
6
|
0.46 (0.37–0.54)
|
0.0
|
0.798
|
11.02
|
0.000
| |
|
Preparation method
|
|
No preparation
|
6
|
0.42 (0.30–0.55)
|
63.4
|
0.018
|
6.72
|
0.000
|
0.04
|
|
Swim up
|
3
|
0.27 (0.01–0.53)
|
80.6
|
0.006
|
2.01
|
0.044
| |
|
Discontinuous gradient
|
2
|
0.42 (0.23–0.60)
|
0.0
|
0.893
|
4.41
|
0.000
| |
|
AE assay method
|
|
Fluorometry
|
|
MAb 4D4-proacrosin
|
1
|
0.49 (0.27–0.70)
|
–
|
–
|
4.43
|
0.000
|
< 0.001
|
|
Spectrophotometry
|
|
Spectrophotometry/BAPNA
|
5
|
0.44 (0.35–0.54)
|
0.0
|
0.618
|
9.08
|
0.000
| |
|
Accu-Sperm spectrophotometry/BAPNA
|
2
|
0.09 (−0.05–0.23)
|
0.0
|
0.352
|
1.22
|
0.223
| |
|
Substrate assay
|
|
Acrosin target with gelatine substrate assay
|
2
|
0.43 (0.30–0.56)
|
0.0
|
0.997
|
6.51
|
0.000
| |
|
Hyaluronidase target with agar/hyaluronic acid mixture substrate assay
|
1
|
0.54 (0.43–0.65)
|
–
|
–
|
9.70
|
0.000
| |
- Rs spearman correlation coefficient, CI confidence interval, BAPNA N-α-benzoyl-dl-arginine-p-nitroanilide
- aComparison between subgroups with the Q test for heterogeneity
- bNot calculated
