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Table 2 Characteristics of datasets that addressed the relationship between the AE levels and FR

From: Human sperm acrosome function assays are predictive of fertilization rate in vitro: a retrospective cohort study and meta-analysis

First author and year Country Design N Preparation method Storage method AE assay method FR cut-off value AE cut-off value Outcome
Kruger 1988 [50] USA Prospective cohort 60 No preparation Cryopreservation Spectrophotometry/BAEE >  0% vs. = 0%  
Kennedy 1989 [6] USA Prospective cohort 35 No preparation Fresh Spectrophotometry/BAPNA >  0% vs. = 0%
/= 0%
<  25μIU/106 spermatozoa /
Tummon 1991a [7] USA Prospective cohort 87 No preparation Fresh Accu-Sperm Spectrophotometry/BAPNA >  0% vs. = 0%
/= 0%
<  4.5 /
Tummon 1991b [7] USA Prospective cohort 87 Swim up Fresh Accu-Sperm Spectrophotometry/BAPNA >  0% vs. = 0%  
Albert 1992a [4] France Prospective case-control 50 No preparation Fresh MAb 4D4-proacrosin Wholea
/>  0% vs. = 0%
/= 0%
≤ 50% //
Albert 1992b [4] France Prospective case-control 50 Swim up Fresh MAb 4D4-proacrosin >  0% vs. = 0%  
Senn 1992a [3] Switzerland Prospective cohort 151 Discontinuous gradient Fresh PAb-acrosin >  0% vs. =0%
/= 0%
<  60% /
Senn 1992b [3] Switzerland Prospective cohort 148 Discontinuous gradient Fresh PAb-hyaluronidase >  0% vs. = 0%
/= 0%
<  80% /
Senn 1992c [3] Switzerland Prospective cohort 96 Discontinuous gradient Fresh Spectrophotometry/BAPNA = 0% <  30 μIU/106 spermatozoa
De Jonge 1993 [8] USA Prospective cohort 21/60 No preparation Fresh Spectrophotometry/BAPNA >  70%b
/≥ 70% vs. <  70%
<  12.8 μIU/106 spermatozoa /
Sharma 1993a [54] UK Prospective cohort 46 Swim up Fresh Spectrophotometry/BAPNA Whole  
Sharma 1993b [54] UK Prospective cohort 31 Discontinuous gradient Fresh Spectrophotometry/BAPNA Whole  
Sharma 1993c [54] UK Prospective cohort 25 α-Chymotrypsin Fresh Spectrophotometry
/BAPNA
Whole  
Bartoov 1994 [57] Israel Prospective case-control 59 No preparation Fresh Spectrophotometry
/BAPNA
Whole
/>  50% vs. = 0%/= 0%
≤ 54 μIU/106 spermatozoa //
Sofikitis 1994 [51] USA Prospective cohort 41 No preparation Fresh Accu-Sperm
Spectrophotometry
/BAPNA
> 0% vs. = 0%  
Yang 1994a [9] China Prospective cohort 95 No preparation Fresh Accu-Sperm
Spectrophotometry
/BAPNA
Whole
/>  0% vs. = 0%
  /
Yang 1994b [9] China Prospective cohort 95 Swim up Fresh Accu-Sperm
Spectrophotometry
/BAPNA
Whole
/>  0% vs. = 0%
  /
Abdul-Aziz 1995 [19] Canada Prospective cohort 160 No preparation Fresh Hyaluronidase target with agar/hyaluronic acid mixture substrate Whole ≤ 3 mm
Henkel 1995
[16]
Germany Prospective cohort 110 Swim up Fresh Acrosin target with gelation substrate assay whole/
≤ 50%
<  6 /
Menkveld 1996 [55] Netherlands Prospective cohort 33 No preparation Fresh Spectrophotometry
/BAPNA
whole
/≥ 50% vs. <  50%/<  50%
≤ 18 μIU/106 spermatozoa //
Yie 1996
[53]
Canada Prospective cohort 259 Swim up Cryopreservation Spectrophotometry
/BAPNA
> 0% vs. 0%
/= 0%
≤ 15 μIU/
μgDNA
/
Langlois 2005
[56]
Belgium Prospective cohort 107 No preparation Fresh Spectrophotometry
/BAPNA
whole
/≥ 50% vs. <  50%/<  50%
<  25 μIU/106 spermatozoa //
Tavalaee 2007 [18] Iran Prospective cohort 48 Discontinuous gradient Fresh Acrosin target with gelation substrate assay whole
/<  50%
<  60% /
  1. AE acrosomal enzyme, FR fertilization rate, BAEE N-benzoyl-l-arginine ethyl ester, BAPNA N-α-benzoyl-dl-arginine-p-nitroanilide
  2. aAll infertile patients undergoing in vitro fertilization (IVF) therapy
  3. bInfertile patients for whom ≥70% fertilization was achieved by IVF
  4. spearman correlation coefficient; AE levels for higher and lower FR; binary diagnostic accuracy data as 2 × 2 tables