Table 1 Primer sequence for reverse transcription PCR
From: Peroxiredoxin I maintains luteal function by regulating unfolded protein response
Target | Accession numbers | Primer | Sequence reported 5′-3′ | Tm°C | length (bp) |
|---|---|---|---|---|---|
3β-hsd | NM_008293.3 | Forward:5’ | ACTGCAGGAGGTCAGAGCT | 55 | 401 |
Reverse:3’ | GCCAGTAACACACAGAATACC | ||||
Prdx1 | NM_011034.4 | Forward:5’ | CACCCAAGAAACAAGGAGGA | 53.5 | 343 |
Reverse:3’ | TGGTCCAGTGCTCACTTCTG | ||||
Star | NM_011485.4 | Forward:5’ | GAAAAGACACGGTCATCACT | 56 | 262 |
Reverse:3’ | CCGTGTCTTTTCCAATCCTC | ||||
P450scc (Cyp11a1) | AF195119.1 | Forward:5’ | GCTGGAAGGTGTAGCTCAGG | 57 | 224 |
Reverse:3’ | CACTGGTGTGGAACATCTGG | ||||
Gapdh | BC145810.1 | Forward:5’ | ACCACAGTCCATGCCATCAC | 55 | 452 |
Reverse:3’ | TCCACCACCCTGTTGCTGTA |