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Fig. 6 | Reproductive Biology and Endocrinology

Fig. 6

From: Peroxiredoxin I maintains luteal function by regulating unfolded protein response

Fig. 6

Deceased serum progesterone, steroidogenesis enzymes, and increased activation of UPR signaling in response to ER stress in Prdx1 K/O mice are restored by injecting NAC, a ROS scavenger. a Serum progesterone levels of PRDX1 were measured using a progesterone kit after NAC (1.0 μg/g) i.p injection. b RT-PCR analysis of Prdx1 and three major genes encoding steroidogenesis enzymes (3β-HSD, StAR, and P450scc). c The levels of PRDX1, UPR signaling-related proteins (GRP78/BIP and p50ATF5), and ER stress-mediated apoptotic factors (CHOP) were determined in the CL tissue of Prdx1 K/O mice by western blot analysis after NAC (1.0 μg/g) i.p injection. The relative mRNA/protein levels were normalized to those of GAPDH/β-actin controls. The histogram values of densitometry analysis were obtained using Image J software. The data are representative of at least three independent experiments conducted in triplicate and shown as means ± SEM (3–5 mice/group). * P < 0.05, **P < 0.01, and ***P < 0.001; Dunnett’s multiple comparison test, compared to 48 h after PMSG/hCG injection

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