Fig. 5

Protein levels of UPR signal activation and ER stress-mediated apoptosis in CL functional stage were induced in the CL tissue of Prdx1 K/O mice. a Western blot analysis to evaluate the levels of PRDX1 and UPR signaling-related proteins (GRP78/BIP, p-EIF2α, EIF2α, p50ATF5, and p-IRE1) and (b) ER stress-mediated apoptotic factors (CHOP, p-JNK, JNK, and caspase-3) in the CL. The relative levels of these proteins were obtained after normalization with β-actin levels. The histogram values of densitometry analysis were obtained using the Image J software. The bars represent the means of two independent experiments ± SEM (4–5 mice/per group) * P < 0.05, **P < 0.01, and ***P < 0.001; two-tailed Student’s t-test compared to WT mice. c IHC staining for UPR markers (p-EIF2α and ATF6) and ER stress apoptosis marker (cleaved caspase-3) in CL tissue from ovary of Prdx1 K/O mice. Scale bar = 200 μm