Fig. 4

The CL number, serum progesterone, 3β-HSD, and P450ssc expression were decreased in Prdx1 K/O mice. a We performed genotyping of PRDX1 by PCR in the tail of Prdx1 K/O mice. b CL number in the ovary of Prdx1 K/O mice was observed by hematoxylin and eosin (H & E) staining. c Serum progesterone levels were measured using a progesterone kit in Prdx1 K/O mice. d The levels of the steroidogenic enzymes 3β-HSD and P450SCC were determined in CL tissue by western blot analysis. The relative levels of these proteins were obtained after normalization with β-actin levels. The histogram values of densitometry analysis were obtained using the Image J software. The bars represent the mean of two independent experiments means ± SEM (4–5 mice/group) * P < 0.05, **P < 0.01, and ***P < 0.001; two-tailed Student’s t-test compared to WT mice