Fig. 3

Increased PRDX1 expression by injection of ER-stress inducer (Tm) in the CL tissue was recovered by ER-stress inhibitor (TUDCA) or ROS scavenger (NAC). a Schematic diagram showing the time taken to reach peak progesterone levels in CL 48 h after PMSG/hCG injection. b Protein levels of PRDX1 and 3β-HSD were measured by western blot analysis in different sampling times of the luteal phase. c Schematic diagram showing the timing of ER stress-regulator and ROS scavenger injection and sample collection. d PRDX1 levels in selected CL tissues were measured using western blot analysis in the presence of the ER stress inducer (Tm) (0.5 μg/g body weight) or ER stress inhibitor (TUDCA) (0.5 μg/g body weight). And ER stress inhibitor (TUDCA) (0.5 μg/g body weight) or ROS scavenger (NAC) (1.0 μg/g body weight) after pre-treatment with the ER stress inducer (0.5 μg/g Tm), respectively. 3β-HSD and PRDX1 levels were normalized to that of β-actin. The histogram represents values densitometry analysis was performed using the Image J software (NIH). The bar graphs represent the least-squares means ± SEM (4–5 mice/group) of three independent experiments. * P < 0.05, **P < 0.01, and ***P < 0.001