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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Structure of preantral follicles, oxidative status and developmental competence of in vitro matured oocytes after ovary storage at 4 °C in the domestic cat model

Fig. 3

a. Representative images of fluorescent immunodetection of γH2AX phosphorylation: follicle-enclosed oocytes with nuclei without bright foci (A, C) or expressing green fluorescent foci (B, D, white arrows). b Percentage (mean ± S.E.M) of preantral follicles with 3 or more foci/oocyte after different time of ovary storage at 4 °C. Nuclei were stained with propidium iodide (red). Scale bar =10 μm. Different superscripts (a, b) among storage times indicate P < 0.05. n = number of preantral follicles analyzed per storage time

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