Fig. 4
Transcripts levels of the candidate genes of PWRN2-miR-92b-3p-TMEM120B ceRNA network according to oocyte nuclear maturity in patients with PCOS (n = 30). The transcript levels of miR-92b-3p (a), PWRN2 (b) and TMEM120B (c) were detected by qRT-PCR. d Relationship between the expression levels of PWRN2 and TMEM120B. The signal intensity for the genes is shown on the y-axis in arbitrary units determined by qRT-PCR analysis. GAPDH was used as internal control for PWRN2 and TMEM120B, while U6 was used as internal control for miR-92b-3p. * indicates a significant difference in gene expression between CC categories (** P < 0.01, * P < 0.05). The results were presented as means ± SEM. CCMI/GV: cumulus cells from oocyte at the MI or GV stage; CCMII: cumulus cells from oocyte at the MII stage