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Fig. 2 | Reproductive Biology and Endocrinology

Fig. 2

From: Construction and analysis of a lncRNA (PWRN2)-mediated ceRNA network reveal its potential roles in oocyte nuclear maturation of patients with PCOS

Fig. 2

Expression profiles of lncRNAs and mRNAs in KGN/shPWRN2 cell lines. a Relative expression of PWRN2 mRNA was examined in KGN cells infected with different lentiviral shRNAs (LV-PWRN2-homo-502, LV-PWRN2-homo-1574, LV-PWRN2-homo-1261, or LV negative control) using qRT-PCR analysis with GAPDH as an endogenous reference. The KGN cells treated with different lentiviral shRNAs are shown on the x-axis, and the relative change of PWRN2/GAPDH is shown on the y-axis. Each set of qRT-PCR reactions was repeated at least three times. The results are presented as mean ± SEM. ** indicates P < 0.01. b Cluster of lncRNAs and mRNAs that were dyexpressed in KGN/shPWRN2 cells. From this lncRNA+mRNA microarray data, 176 lncRNAs changed significantly in KGN/shPWRN2 cells compared with those in the control cell lines. Among these lncRNAs, 118 were up-regulated, whereas 58 lncRNAs were down-regulated; and 131 mRNAs changed significantly in the KGN/shPWRN2 cell lines. Among these, 84 mRNAs were up-regulated, and 47 mRNAs were down-regulated. The supervised hierarchical clustering of genes overexpressed in KGN/shPWRN2 cells is shown. Distinct signatures were observed in the KGN/shPWRN2 cells. The value of each gene was adjusted with a median-centring algorithm on a log scale; the colours indicate the relative gene expression in the red-green heat map. Pure black indicates 0.00 and represents no change in the median gene expression levels in all samples. Pure green indicates − 2.00 and represents lower expression. Pure red indicates + 2.00 and relatively higher expression

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