Fig. 8

The morphology of NEC treated with E2, TGF-β1, Melatonin, DAPT, a combination of E2 and Melatonin, or a combination of E2 and DAPT for 48 h. The cells were observed using phase contrast microscopy at 400× magnification. Scale bar: 20 μm.E2: 17β-estradiol; MLT: Melatonin