Fig. 6

The expression of EMT and Notch signaling pathway-related markers in NEC. NEC were treated with different drugs for 72 h. a The protein expression of Notch1 (NICD), E-cadherin, N-cadherin, Vimentin, Slug, Snail, Numb and Actin were determined by western blot. β-actin was used as a loading control. b The ratios of Notch1/ E-cadherin/ N-cadherin/Vimentin/Slug/ Snail/Numb to β-actin were analyzed. Data are presented as the mean ± SD. E2: 17β-estradiol; MLT: melatonin. *p < 0.05, ** p < 0.01 vs. untreated cells. #p < 0.05, ##p < 0.01 vs. E2-treated cells