Comparison of the enzymatic efficiency of Liberase TM and tumor dissociation enzyme: effect on the viability of cells digested from fresh and cryopreserved human ovarian cortex

Table 1 Total number of follicles isolated from frozen/thawed ovarian tissues after enzymatic digestion with different kinds of enzymes in 14 experiments

Number of patient	Patient age (years)	Treatment of ovarian cortex	Enzymatic digestion (follicles number)		Volume of piece (mm³)	Byopsy weight (g)			Follicle density/(mm³)
Number of patient	Patient age (years)	Treatment of ovarian cortex	TDE**	Liberase TM***	Volume of piece (mm³)	Whole byopsy weight	Byopsy for TDE digestion	Byopsy for Liberase TM digestion	Follicle density/(mm³)
1	26	frozen	10	5	20	0.0192	0.0090	0.0102	8
2	29	frozen	15	7	30	0.0738	0.0378	0.0360	7
3	33	frozen	23	6	45	0.0941	0.0460	0.0482	7
4	22	frozen	43	20	40	0.0973	0.0479	0.0494	12
5	31	frozen	12	9	22	0.0257	0.0125	0.0132	7
6	39	frozen	14	7	36	0.0854	0.0440	0.0414	5
7	23	frozen	55	13	180	0.2805	0.1396	0.1409	5
8	29	frozen	34	6	55	0.1342	0.0665	0.0677	8
9	34	frozen	125	84	165	0.2348	0.116	0.1188	10
10	20	frozen	321	168	144	0.2220	0.116	0.106	24
11	34	frozen	216	115	157	0.2253	0.1114	0.1139	17
12	32	fresh	44	16	200	0.4592	0.2310	0.2286	4
13	34	fresh	30	12	210	0.5775	0.2905	0.2870	2
14	28	fresh	48	19	55	0.1401	0.0691	0.0710	11
Total	–	–	990	487	–	–
Mean ± SD			70.7 ± 87.7*	34.8 ± 48.7	81.2 ± 71.8	0.18 ± 0.17	0.096 ± 0.1	0.095 ± 0.1	9.1 ± 5.5

*asterisk corresponding the statistical (P < 0.01)
**TDE (Dr. Christian Sartori, Labour, Hamburg, Germany) is a commercial Enzyme-cocktail (Patent Nr. WO 2006031867 A2, 2004)
***Liberase TM Research grade belongs to the group of Liberase Research Grade Purified Enzyme Blends with reduced endotoxine levels and are mixtures of highly purified Collagenase I and Collagenase II, and with a medium concentration of Thermolysin

ISSN: 1477-7827