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Table 1 Total number of follicles isolated from frozen/thawed ovarian tissues after enzymatic digestion with different kinds of enzymes in 14 experiments

From: Comparison of the enzymatic efficiency of Liberase TM and tumor dissociation enzyme: effect on the viability of cells digested from fresh and cryopreserved human ovarian cortex

Number of patient Patient age (years) Treatment of ovarian cortex Enzymatic digestion (follicles number) Volume of piece (mm3) Byopsy weight (g) Follicle density/(mm3)
TDE** Liberase TM*** Whole byopsy weight Byopsy for TDE digestion Byopsy for Liberase TM digestion
1 26 frozen 10 5 20 0.0192 0.0090 0.0102 8
2 29 frozen 15 7 30 0.0738 0.0378 0.0360 7
3 33 frozen 23 6 45 0.0941 0.0460 0.0482 7
4 22 frozen 43 20 40 0.0973 0.0479 0.0494 12
5 31 frozen 12 9 22 0.0257 0.0125 0.0132 7
6 39 frozen 14 7 36 0.0854 0.0440 0.0414 5
7 23 frozen 55 13 180 0.2805 0.1396 0.1409 5
8 29 frozen 34 6 55 0.1342 0.0665 0.0677 8
9 34 frozen 125 84 165 0.2348 0.116 0.1188 10
10 20 frozen 321 168 144 0.2220 0.116 0.106 24
11 34 frozen 216 115 157 0.2253 0.1114 0.1139 17
12 32 fresh 44 16 200 0.4592 0.2310 0.2286 4
13 34 fresh 30 12 210 0.5775 0.2905 0.2870 2
14 28 fresh 48 19 55 0.1401 0.0691 0.0710 11
Total 990 487    
Mean ± SD    70.7 ± 87.7* 34.8 ± 48.7 81.2 ± 71.8 0.18 ± 0.17 0.096 ± 0.1 0.095 ± 0.1 9.1 ± 5.5
  1. *asterisk corresponding the statistical (P < 0.01)
  2. **TDE (Dr. Christian Sartori, Labour, Hamburg, Germany) is a commercial Enzyme-cocktail (Patent Nr. WO 2006031867 A2, 2004)
  3. ***Liberase TM Research grade belongs to the group of Liberase Research Grade Purified Enzyme Blends with reduced endotoxine levels and are mixtures of highly purified Collagenase I and Collagenase II, and with a medium concentration of Thermolysin