Table 4 Gene ontology of predicted targets for heat-sensitive miRNAs found in pachytene spermatocytes
From: The thermo-sensitive gene expression signatures of spermatogenesis
| Â | No of genes | Predicted targets |
|---|---|---|
Molecular functions | ||
 Binding | 15 | Taf9b, Syt4, Cpeb1, Upf2, Arhgef2, Plch1, Net1, Arid3b, Enc1, Pole4, Impad1, Rfx7, Camk1d, Aph1a, Nfyb |
 Catalytic activity | 22 | Atp11c, Upf2, Dusp10, Arhgef2, Plch1, Mtor, Net1, Tmtc3, Casp9, Cnot8, Kbtbd8, Pole4, Impad1, Tesk2, Camk1d, Mapk8, Map3k14, Aph1a, Map4k3, Acly, Map3k3, Nfyb |
 Receptor activity | 1 | Net1 |
 Signal transducer activity | 2 | Dusp10, Map4k3 |
 Structural molecule activity | 1 | Enc1 |
 Translation regulator activity | 1 | Cpeb1 |
 Transporter activity | 3 | Atp11c, Cacna1a, Slc30a4 |
Biological processes | ||
 Biological adhesion | 3 | Arhgef2, Net1, Net1 |
 Biological regulation | 9 | Atp11c, Syt4, Cacna1a, Dusp10, Casp9, Slc30a4, Map3k14, Map4k3, Map3k3 |
 Cellular component organization or biogenesis | 3 | Atp11c, Syt4, Tesk2 |
 Cellular process | 28 | Atp11c, Syt4, Cpeb1, Cacna1a, Dusp10, Arhgef2, Plch1,Mtor, Net1, Tmtc3, Net1, Cltc, Casp9, Cnot8, Enc1, Slc30a4, Kbtbd8, Smurf1, Impad1, Rfx7, Tesk2, Camk1d, Gphn, Mapk8, Map3k14, Map4k3, Map3k3, Nfyb |
 Developmental process | 11 | Lmtk2, Arhgef2, Epha4, Net1, Net1, Casp9, Enc1, Tesk2, Map3k14, Map4k3, Map3k3 |
 Immune system process | 2 | Tesk2, Mapk8 |
 Localization | 2 | Atp11c, Cltc |
 Metabolic process | 23 | Taf9b, Atp11c, Cpeb1, Upf2, Dusp10, Plch1, Mtor, Tmtc3, Arid3b, Cnot8, Kbtbd8, Smurf1, Pole4, Impad1, Rfx7, Tesk2, Gphn, Map3k14, Aph1a, Map4k3, Acly, Map3k3, Nfyb |
 Multicellular organismal process | 3 | Syt4, Net1, Cltc |
 Reproduction | 1 | Tesk2 |
 Response to stimulus | 11 | Taf9b, Dusp10, Mtor, Casp9, Slc30a4, Smurf1, Tesk2, Mapk8, Map3k14, Map4k3, Map3k3 |
Cellular components | ||
 Cell part | 16 | Atp11c, Cpeb1, Cltc, Casp9, Cnot8, Enc1, Kbtbd8, Smurf1, Pole4, Impad1, Rfx7, Camk1d, Gphn, Map3k14, Map4k3, Map3k3 |
 Extracellular matrix | 1 | Net1 |
 Extracellular region | 1 | Net1 |
 Macromolecular complex | 4 | Cpeb1, Cltc, Cnot8, Kbtbd8 |
 Membrane | 3 | Atp11c, Syt4, Cacna1a |
 Organelle | 4 | Atp11c, Cpeb1, Pole4, Rfx7 |
Protein classes | ||
 Calcium binding protein | 1 | Plch1 |
 Cytoskeletal protein | 1 | Enc1 |
 Enzyme modulator | 5 | Arhgef2,Plch1,Net1,Casp9,Aph1a |
 Extracellular matrix protein | 1 | Net1 |
 Hydrolase | 4 | Atp11c,Plch1,Casp9,Impad1 |
 Ligase | 2 | Smurf1,Acly |
 Lyase | 1 | Acly |
 Membrane traffic protein | 2 | Syt4,Cltc |
 Nucleic acid binding | 8 | Taf9b,Cpeb1,Upf2,Mtor,Arid3b, Pole4, Rfx7, Nfyb |
 Receptor | 1 | Net1 |
 Signalling molecule | 1 | Plch1 |
 Transcription factor | 6 | Taf9b,Arid3b, Cnot8, Pole4, Rfx7, Nfyb |
 Transferase | 6 | Mtor, Tmtc3, Tesk2, Camk1d, Mapk8, Acly |
 Transporter | 3 | Atp11c, Cacna1a, Slc30a4 |
Pathways | ||
 5HT2 type receptor mediated signaling pathway | 1 | Plch1 |
 Alzheimer disease-amyloid secretase pathway | 2 | Mapk8, Aph1a |
 Alzheimer disease-presenilin pathway | 1 | Aph1a |
 Angiogenesis | 2 | Casp9, Mapk8 |
 Apoptosis signaling pathway | 4 | Casp9, Mapk8, Map3k14, Map4k3 |
 Axon guidance mediated by Slit/Robo | 1 | Net1 |
 Axon guidance mediated by netrin | 1 | Net1 |
 B cell activation | 2 | Mapk8, Map3k3 |
 CCKR signaling map | 2 | Mapk8, Map3k14 |
 EGF receptor signaling pathway | 2 | Mapk8, Map3k3 |
 Endogenous cannabinoid signaling | 1 | Cacna1a |
 FAS signaling pathway | 2 | Casp9, Mapk8 |
 FGF signaling pathway | 2 | Mapk8, Map3k3 |
 GABA-B receptor II signaling  General transcription regulation | 1 | Cacna1a |
 Gonadotropin-releasing hormone receptor pathway | 6 | Syt4, Mapk8, Map3k3, Map3k14, Map4k3, Nfyb |
 Heterotrimeric G-protein signaling pathway-Gi alpha and Gs alpha mediated pathway | 1 | Cltc |
 Heterotrimeric G-protein signaling pathway-Gq alpha and Go alpha mediated pathway | 2 | Cacna1a, Cltc |
 Histamine H1 receptor mediated signaling pathway | 1 | Plch1 |
 Hypoxia response via HIF activation | 1 | Mtor |
 Inflammation mediated by chemokine and cytokine signaling pathway | 1 | Plch1 |
 Integrin signalling pathway | 2 | Mapk8, Map3k3 |
 Interferon-gamma signaling pathway | 1 | Mapk8 |
 Interleukin signaling pathway | 1 | Mtor |
 Ionotropic glutamate receptor pathway | 1 | Cacna1a |
 Metabotropic glutamate receptor group II pathway | 1 | Cacna1a |
 Metabotropic glutamate receptor group III pathway | 1 | Cacna1a |
 Notch signaling pathway | 1 | Aph1a |
 Oxidative stress response | 2 | Dusp10, Mapk8 |
 Oxytocin receptor mediated signaling pathway | 1 | Plch1 |
 PDGF signaling pathway | 2 | Mtor, Mapk8 |
 PI3 kinase pathway | 1 | Casp9 |
 Parkinson disease | 1 | Mapk8 |
 Pyruvate metabolism | 1 | Acly |
 Ras Pathway | 1 | Mapk8 |
 T cell activation | 1 | Mapk8 |
 TGF-beta signaling pathway | 2 | Smurf1, Mapk8 |
 Thyrotropin-releasing hormone receptor signaling pathway | 2 | Cacna1a Plch1 |
 Toll receptor signaling pathway | 1 | Mapk8 |
 Transcription regulation by bZIP transcription factor | 1 | Taf9b |
 Ubiquitin proteasome pathway | 1 | Smurf1 |
 VEGF signaling pathway | 1 | Casp9 |
 p38 MAPK pathway | 1 | Dusp10 |
 p53 pathway by glucose deprivation | 1 | Mtor |