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Fig. 4 | Reproductive Biology and Endocrinology

Fig. 4

From: Exogenous leptin affects sperm parameters and impairs blood testis barrier integrity in adult male mice

Fig. 4

Leptin directly reduced the expression of TJ-associated proteins in vitro, and inhibitors of leptin signaling mediators abolished leptin’s effect to different degrees. a detection of OB-R in TM4 cells, the bands of 471 bp and 281 bp corresponded to OB-R and β-Actin, respectively. b western blot analysis of claudin 5, occludin and ZO-1 in TM4 cells after treated with 0 (control), 10 or 100 nM leptin for 48 h. c densitometric analysis for immunopositive bands of claudin 5, occludin and ZO-1 in TM4 cells. d western blot analysis of claudin 5, occludin and ZO-1 in TM4 cells, cells were treated with 100 nM leptin or pre-treated with different inhibitors following a 100 nM leptin treatment. e densitometric analysis for immunopositive bands of claudin 5, occludin and ZO-1 in inhibitor assay. Data are expressed as mean ± SD, n = 5. * versus control, # versus 100 nM leptin, p < 0.05

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