Fig. 2
Evaluation of testicular cell apoptosis and BTB integrity. a the number of TUNEL positive nuclei per seminiferous tubule. Data are expressed as mean ± SD, n = 3. * versus control, p < 0.05. b TUNEL positive nuclei (green) which indicated apoptosis were mainly localized in germ cells in seminiferous tubules. Cell nuclei were stained with DAPI (blue). c biotin (red) only passed through the BTB and accumulated in the adluminal compartments of seminiferous tubules in 3 mg/kg leptin-treated mice. Cell nuclei were stained with DAPI (blue). d proportion of seminiferous tubules that have the biotin in the adluminal compartments in all observed seminiferous tubules at stage VIII and other stages. n = 4. χ2 = 10.323, p < 0.05