Table 1 Primers used in quantitative real-time PCR
Gene | Accession number | Primer sequences (5′–3′) |
|---|---|---|
Star | NM_011485.4 | Upstream: CGGGTCTTGGGTCCTGGATTGT |
Downstream: GCTGCCCTCGCTCACCTTAAG | ||
Cyp19a1 | NM_007810.3 | Upstream: TCGTCGCAGAGTATCCAGAGGT |
Downstream: CGCATGACCAAGTCCACAACAG | ||
Cyp11a1 | NM_019779.3 | Upstream: GCTCAACCTGCCTCCAGACTTC |
Downstream: CCTCCTGCCAGCATCTCGGTAA | ||
Hsd17b7 | NM _010476.3 | Upstream: CTGTGACACCGTACAACGGA |
Downstream: GCTCGGGTGATCCGATTTCT | ||
Jak2 | NM_008413.3 | Upstream: AGATGGGAAGGGAAGGGTGTGG |
Downstream: AAGAGGGAGCAGCACGAAGGAT | ||
Stat3 | NM_213659.3 | Upstream: TCGGTTGGAGGTGTGAGGTAGG |
Downstream: GTGGGAAAGGAAGGCAGGTTGA | ||
Stat5 | NM_001164062.1 | Upstream: CAGAGTCGGTGACGGAGGAGAA |
Downstream: GCACGGTGGCAGTAGCATTGT | ||
Gapdh | NM _001289726.1 | Upstream: GTGAAGGTCGGTGTGAACGGATT |
Downstream: GGTCTCGCTCCTGGAAGATGGT |