Fig. 3

Hematoxylin and eosin staining of brown and white adipocytes and expression of mRNA for steroid receptors in brown and white adipose tissues of CTL and DHEA-treated wild type and Gpr1-null mice. A Adipocyte diameters measured in hematoxylin and eosin-stained sections (bar = 50 um) and levels of steroid receptor (B-E) and Ucp1 mRNAs in scapular brown adipose tissue (B), mesenteric white adipose tissue (C), inguinal white adipose tissue (D), and gonadal white adipose tissue (E). Values for diameters represent means ± SEM (n = 6 mice per group). Significance of differences between groups was determined using an unpaired Student’s t test. (a) p, DHEA-treated vs. control wild type mice; (b) p, DHEA-treated vs. control Gpr1-null mice; (c) p, control Gpr1-null mice vs. control wild type mice; (d) p, DHEA-treated Gpr1 null mice vs. DHEA-treated wild type mice