The preclinical evaluation of immunocontraceptive vaccines based on canine zona pellucida 3 (cZP3) in a mouse model

Table 1 Contraceptive effects of the recombinant proteins in the first mating test

Antigen	Fertility rate (%)^a	Mean litter size^b	Average Abs levels	P ^c
Adjuvant	100% (10/10)	7 ± 0.516	0.050 ± 0.004^d
Adjuvant	100% (10/10)	7 ± 0.516	0.183 ± 0.02^e
OGZ	10% (1/10)	0.4 ± 0.4	2.092 ±0 .162 ^d	***
OGZ	10% (1/10)	0.4 ± 0.4	1.095 ± 0.056 ^e	***
OZ	50% (5/10)	2.3 ± 0.883	2.138 ±0 .334 ^d	**
cZP3	50% (5/10)	2.2 ± 0.772	2.134 ±0 .103 ^d	**
mZP3	40% (4/10)	2 ± 0.907	2.215 ±0 .156 ^d	***

^aFertility rate: number of the fertile mice/total number of the mated mice in each group
^bMean litter size of mice: total number of pups in each group/total number of the mated mice in each group
^cMean litter size in each group vs adjuvant group;* represents P < 0.05; ** represents P < 0.01; *** represents P < 0.005
^dAverage Abs levels of cZP3
^eAverage Abs levels of GnRH

ISSN: 1477-7827