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Table 2 Fatty acid composition from total lipids of bovine granulosa cells after 15 h treatment with DHA

From: Docosahexaenoic acid (DHA) effects on proliferation and steroidogenesis of bovine granulosa cells

Fatty acids (mole % of total fatty acids) Control a DHA1 μM DHA 10 μM DHA 50 μM
n = 5 n = 4 n = 4 n = 3
Saturates
 16:0 31.50 ± 7.48 40.16 ± 9.38 44.12 ± 11.60 48.45 ± 7.44
 18:0 10.68 ± 2.25 13.36 ± 3.03 15.04 ± 3.89 16.24 ± 2.60
Monounsaturates
 16:1 n-7 2.32 ± 0.55 3.03 ± 0.72 3.33 ± 0.91 3.52 ± 0.68
 18:1 n-7 8.93 ± 2.17 11.51 ± 2.85 12.36 ± 3.72 12.22 ± 3.01
 18:1 n-9 cis 29.99 ± 7.18 38.91 ± 9.08 42.49 ± 11.58 43.03 ± 7.86
n-6 PUFA
 18:2 n-6 cis 6.05 ± 1.29 7.87 ± 1.81 8.59 ± 2.33 8.41 ± 2.07
 20:4 n-6 8.85 ± 2.12 11.87 ± 3.08 13.02 ± 3.99 11.24 ± 4.10
n-3 PUFA
 18:3 n-3 0.51 ± 0.12 0.66 ± 0.15 0.74 ± 0.18 0.67 ± 0.17
 20:5 n-3 0.98 ± 0.25 1.34 ± 0.31 1.60 ± 0.38 1.37 ± 0.42
 22:5 n-3 7.03 ± 1.72 9.33 ± 2.28 10.04 ± 2.90 7.79 ± 3.31
 22:6 n-3 (DHA) 0.84 ± 0.16 2.17 ± 0.04 * 10.27 ± 0.11 * 23.05 ± 6.58 *
  1. Granulosa cells (GC) (2.5 × 105 viable cells/250  μL media/well) were cultured into 48-well dishes in modified McCoy’s 5A media in the presence or absence of DHA (1, 10 or 50 μM). After 15  h culture, supernatants were removed and cells with the same treatment (12 wells) were pooled and stored under nitrogen gas at −80 °C until the total lipid extraction. The fatty acid analysis was performed by gas chromatography by the Plateforme de Lipidomique Fonctionnelle (INSA, Villeurbanne, France). The relative amount of each fatty acid is expressed as the area of each fatty acid peak, relative to the total area for all fatty acids (mole%). The results are expressed as means ± SEM (n = 4 per treatment). a Control corresponds to the serum-free modified McCoy’s 5A medium supplemented with DMSO (1:2000) as carrier solvent of DHA. The prepared mediums with the different concentrations of DHA were all adjusted for DMSO at 1:2000. * indicates a significant difference with control condition (Kruskal-Wallis test with Tukey’s multiple comparison test as post-hoc test, p < 0.05)