Table 1 Primer sequences for real-time reverse transcription–polymerase chain reaction
From: Docosahexaenoic acid (DHA) effects on proliferation and steroidogenesis of bovine granulosa cells
Abbrev. | Gene | Accession no. | Forward primer | Reverse primer | bp | E % |
|---|---|---|---|---|---|---|
FFAR4 | Free fatty acid receptor 4 | XM_865266 | GGGTTCCTTTTCGATGTGAA | GCCGTGACTCTTTGGAGAAG | 166 | 94 |
GPX4 | Glutathione peroxidase 4 | NM_174770 | CGATACGCCGAGTGTGGTTTAC | ACAGCCGTTCTTGTCAATGAGG | 261 | 96 |
GLUT1 | Solute carrier family 2 (facilitated glucose transporter), member 1 | NM_174602 | CTGATCCTGGGTCGCTTCAT | ACGTACATGGGCACAAAACCA | 68 | 113 |
NFkB | Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 | NM_001076409.1 | GCACCACTTATGACGGGACT | CCATGTCCAGAGGAGTGGTT | 195 | 89 |
PPARA | Peroxisome proliferator-activated receptor alpha | NM_001034036.1 | CCTACGGGAATGGCTTCATA | GCACAATACCCTCCTGCATT | 219 | 97 |
PPARG | Peroxisome proliferator-activated receptor gamma | Y12419/Y12420 | CCCTGGCAAAGCATTTGTAT | ACTGACACCCCTGGAAGATG | 222 | 88 |
RPL19 | Ribosomal protein L19 | BC102223 | AATCGCCAATGCCAACTC | CCCTTTCGCTTACCTATACC | 156 | 94 |
RPS9 | Ribosomal protein S9 | BC148016 | GGAGACCCTTCGAGAAGTCC | GGGCATTACCTTCGAACAGA | 180 | 100 |
SREBF1 | Sterol regulatory element binding transcription factor 1 | AB355703.1 | ACCGCTCTTCCATCAATGAC | TTCAGCGATTTGCTTTTGTG | 190 | 97 |