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Fig. 7 | Reproductive Biology and Endocrinology

Fig. 7

From: Docosahexaenoic acid (DHA) effects on proliferation and steroidogenesis of bovine granulosa cells

Fig. 7

Gene expression in bovine granulosa cells before or after 8 h treatment with DHA or TUG-891. Effects of DHA or TUG-891 on mRNA expression of solute carrier family 2 member 1 (GLUT1), glutathione peroxidase 4 (GPX4), nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (NFkB), peroxisome proliferator-activated receptor gamma (PPARG), sterol regulatory element binding transcription factor 1 (SREBF1), free fatty acid receptor 4 (FFAR4) and PPAR alpha (PPARA) were assessed in bovine granulosa cells (GC) before or after 8 h culture in enriched McCoy’s 5A media with various doses of DHA (1, 10, 20 and 50 μM) or TUG-891 (1, 10 and 50 μM), as described in Material and Method section. The chemical DMSO alone (1/2000) was used as a negative control due to its use as a solvent for DHA and TUG-891. Total mRNA was extracted from GC and reverse-transcribed, and real-time RT-PCR was performed. The geometric mean of two housekeeping genes (RPL19- ribosomal protein L19 and RPS9- ribosomal protein S9) was used to normalize gene expression. Results of 10 independent cultures are presented as mean ± SEM. Bars with different superscripts are significantly different (p < 0.05)

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