Skip to main content
Fig. 4 | Reproductive Biology and Endocrinology

Fig. 4

From: Docosahexaenoic acid (DHA) effects on proliferation and steroidogenesis of bovine granulosa cells

Fig. 4

Progesterone secretion from bovine granulosa cells after 48 h treatment with DHA or TUG-891. Effects of DHA or TUG-891 on progesterone secretion were assessed in culture media of bovine granulosa cells cultured for 48 h in enriched McCoy’s 5A media with various doses of DHA (1, 10, 20 and 50 μM) or TUG-891 (1, 10 and 50 μM), as described in Material and Method section. The chemical DMSO alone (1/2000) was used as a negative control due to its use as a solvent for DHA and TUG-891. Progesterone secretion was normalized with the protein concentration in each well and expressed as ng progesterone (P4) per μg protein. Results of 12 independent cultures, with each treatment conducted in quadruplicate, are presented as mean ± SEM. Bars with different superscripts are significantly different (p < 0.05)

Back to article page