Fig. 3

Synthesis of DNA in bovine granulosa cells: ³H-thymidine incorporation after 24 h treatment with DHA or TUG-891. Effects of DHA or TUG-891 on cell proliferation were assessed by measurement of ³H-thymidine incorporation in bovine granulosa cells after 24 h culture in enriched McCoy’s 5A media with various doses of DHA (1, 10, 20 and 50 μM) or TUG-891 (1, 10 and 50 μM), as described in Material and Method section. The chemical DMSO alone (1/2000) was used as a negative control due to its use as a solvent for DHA and TUG-891. The data are expressed as disintegrations per minute. Results represent 13 independent cultures with each treatment conducted in four replicates and are presented as mean ± SEM. Bars with different superscripts are significantly different (p < 0.05)